Abstract of research

EFFECACY OF MONTANIDE GEL INACTIVATED RVF VACCINE IN COMPARISON WITH ALUMINUM HYDROXIDE GEL INACTIVATED ONE

Diana M. Abul Magd1, Mohamed Ebeid2, Abdel-Moneim M. Moustafa2, Eman Mohamed Sayed Shalakamy2, Mohamed G. Abdel Wahab3

Abstract

In this work, 20% concentration of montanide gel adjuvanted inactivated RVF vaccine were prepared, and aluminum hydroxide gel RVF vaccine. The prepared vaccines were safe when inoculated into mice with no deaths or onset of symptoms. The potency of the prepared vaccines in adult mice, revealed that the vaccines gave a protection in mice with ED50/ML (0.0017, and 0.0011 ED50/ML) for the inactivated RVF vaccines with aluminum hydroxide gel, and montanide gel 20%) respectively. Both forms of inactivated RVF vaccine tested for its shelf life during storage 12 months at 4˚c, montanide gel 01 RVF vaccines were within the permissible limit (0.02 ED50/ ml). Results of lymphocytic count cleared that montanide gel inactivated RVF vaccine gave early and higher value of lymphocytic count than Aluminum hydroxide gel inactivated RVF vaccine. The results of estimated interferon γ gene expression revealed that montanide gel induced higher level of interferon γ gene expression than Aluminum hydroxide gel. The previous data clearly showed that montanide gel was highly immunogenic than Aluminum hydroxide gel inactivated one.