Abstract of research

Influence of lactoferrin on immune response in rats intoxicated by diazinon

Assaraj Q.SH1 , Alattar H.A2 , Farid A.S3 , Fararah K.M3

Abstract

This study is done to evaluate the antioxidant effect of Lactoferrin (LF) on liver injury induced by Diazinon (DZN). Seventy five rats were divided into five groups, each group contains 15 rats; control (G1): (no treatments), Lactoferrin (G2): (LF, 100 mg/kg bw orally daily for 6 weeks), diazinon (G3): (DZN, 70 mg/kg bw orally, daily for 4 weeks), DZN +LF (G4): (DZN, 70 mg/kg bw orally, daily for 4 weeks followed by LF, 100 mg/kg bw orally daily for 2 weeks, and (LF +DZN) group (G5): (LF, 100 mg/kg bw orally daily for seven weeks and DZN, 70 mg/kg bw for four weeks only started at the second week of the experiment).Total leukocyte count (TLC) and differential leukocyte count (DLC) were checked in whole blood, cytokine IL-10, total proteins(TP) and protein electrophoresis were checked in serum. In comparison to control group, WBCs found increased significantly in DZN group(P<0.005) and IL-10, TP, albumin, and globulin were found significantly decreased too. In comparison to DZN group, WBCs and IL-10, levels were found significantly increased in (DZN+LF) group (P < 0.05), TP, albumin, and globulin levels were also found significantly increased (P < 0.05), WBCs found significantly decreased in (LF+DZN) group and IL-10, TP, albumin, and globulin were found significantly increased too(P < 0.05) .