Abstract of research

Hepatoprotective Effect of Allopurinol and Garlic Extract against Experimentally – Induced Hepatotoxicity

Aya M. Amer, Ayman S. Farid, Khalid M. Fararah

Abstract

The present study was designed to investigate the hepatoprotective and antioxidant effects of allopurinol and garlic extract against acetaminophen and ethanol induced hepatotoxicity and oxidative stress in rats. Animals were divided into four groups .Group 1 was used as a control. Group2 was administered paracetamol plus ethanol orally for 14 days beginning from day 15 – 28 of the experiment. Group 3 was administered allopurinol orally for 28 days, and from day 15 – 28 of the experiment rats were given paracetamol plus ethanol orally till the end of the experiment. Group 4 was administered garlic extract orally for 28 days, and from day 15– 28 of experiment rats given paracetamol orally plus ethanol till the end of the experiment. Serum was separated and used directly for determination of alanine aminotransferase(ALT), aspartate aminotransferase(AST), albumin, alkaline phosphatase(ALP),bilirubin(BIL) and Tumor necrosis factor –alfa(TNF).Liver samples were taken for histopathological examination, liver malondiadehyde(MDA), Glutathione peroxidase(GPX)and nitric oxide(NO).The result revealed that in group 2 there were significant elevations of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin, Tumor necrosis factor–alfa and liver malondiadehyde and nitricoxide. Furthermore, there were significant decreases in Glutathione peroxidase and albumin compared with control group. Meanwhile, administration of Allopurinol and garlic resulted in significant decrease in all elevated mentioned parameter and increase in Glutathione peroxidase and albumin compared with hepatotoxicity group. Therefore it could be concluded that allopurinol and garlic as strong hepatoprotective and antioxidant effects