Phenotypic and Genotypic Characterization of E. Coli Isolated From Fish and Human

Ashraf A. AbdEl-Tawab, ¹Fatma I. El-Hofy, ¹Adel M.El-Gamal²andHeba O. Ibrahim³

Abstract

The present work aimed to isolate and characterize E. coli bacteria that transmitted from fish to fish handlers in markets and farm workers, for this 200 apparently healthy Oreochromis niloticus were collected randomly from different markets and farms and 50 human skin swabs from sellers at markets and workers at farms were obtained at Kafr El-sheikh governorate, Egypt. These samples were cultured and biochemical characters was studied. Serological identification, antibiogram activity and molecular characterization of some virulence factors were detected. Results, the prevalence of Escherichia coli was 17.5% in Tilapia while its prevalence in human was 24%. Escherichia coli serotypes from fish were O153 :H2, O1 :H7, O125 :H21 and O78. Only one from four isolates of Escherichia coli was positive to eaeA gene and the four isolates were negative to hly gene

Key words

E. coli, biochemical, in vitro sensitivity, virulence genes, transmission, fish, human