EFFECT OF USING DIFFERENT ANTIFOAMS ON TOXIN PRODUCTION OF CLOSTRIDIUM PERFRINGENS TYPE A.

Elham F. El-Sergany, Taha, M.M., El-Helw, H.A., Hala El-Sawy

Abstract

In this study, three types of anti-foaming agent (Polyethylene glycol, simethicone, and charcoal) were used in the process of preparation of C. perfringens type A toxoid. It was found that simethicone and polyethylene glycol when added to toxin producing medium gave a high yield of alpha toxin (α toxin) of C. perfringens type A (120, and 90 Minimum Lethal Dose (MLD) respectively) in comparison to that of medium without anti-foaming agent, which gave alpha toxin with 60 MLD. Medium with added charcoal gave least amount of α toxin (30 MLD). Two formula of C. perfringens type A vaccines were prepared with Simethicone and polyethylene glycol as anti-foams, and the third one was prepared without anti-foam as control one. Testing of these vaccines in rabbits revealed that simethicone as antifoam in toxin producing medium of C. perfringens type A produced high level of toxin production and reduced the dose of vaccine used

Key words

C. perfringens type A, Anti-foam, Vaccine.