Abstract of research

Prevalence and molecular characterization of Pseudomonas species in frozen imported meat

Hemmat M. Ibrahim, Hassan, M. A, Nahla A bou El-Roos, Mohga Abd Elsalam, A.

Abstract

A total of 90 random samples of Frozen American, Brazilian and Indian meat samples (30 of each) were collected from different retail shops and supermarkets in EL-Menofeya Governorate at different production dates. The collected samples were examined bacteriologically and by using PCR tecnique for detection of Pseudomonas species, especially Ps.aeruginosa. There for , Ps. aeruginosa , Ps.alcaligenes , Ps.cepacia , Ps. fluorescence , Ps. proteolytica , Ps. Psychrophila, Ps. putrifaciens, Ps.thermotolerans, Ps. versicularis , Ps. fragi,Ps.Putida Ps.orientalis and Ps.stutzeri were isolated from 2(6.67%), 5 (16.67%), 1 (3.33%), 14 (46.67%), 8 (26.67%), 3(10%), 10 (33.33%),6(20%), 1 (3.33%) and zero for Ps.fragi,Ps.Putida,Ps.orientalis and Ps.stutzeri for frozen American meat samples ,3 (10%) , 8 (26.67%) , 1 (3.33%) , 19 (63.33%),5(16.67%), 7 (23.33%), 13(43.33%), 9 ( 30%) 3(10%) , 2(6.67%) , 1(6.67%) and zero for Ps.orientalis one Ps.stutzeri , for frozen Brazilian meat samples, 6(20%),9(30%),4 (13.33%),25(83.33%),6 (20%), 11(36.67),19(63.33%) , 13(43.33%), 4(13.33%), 3(10%), 3(10%), 1(3.33%) and 2(6.67%) for frozen Indian meat samples, respectively. Regarding to Ps.aeruginosa the total number and percentage of ps.aeruginosa were 2(6.67%) , 3(10%) and 6(20%) for American , Brazilian and Indian frozen meat, respectively with total result of (12.22%). By using PCR technique, the all examined samples by conventional method showed positive result by both of PCR and conventional method to Pseudomonas species. On the other hand of Ps. aeruginosa was detected in one sample of Indian meat by conventional method while it showed negative result by PCR technique.