Abstract of research

The hepato- protective Effect of Stem Cells and Levamisole against Carbon- tetrachloride induced Liver Fibrosis.

Khaled M. M. Fararh, Ayman Samir Farid, Ibtisam Mohammed Azzam and Asmaa Abdel- monsef Sultan

Abstract

This study designed to evaluate the hepatoprotective effect of stem cells only or stem cells and levamisole on experimentally Carbon tetrachloride (CCl4) induced liver damage by evaluation of haematological parameters, biochemical parameters, immunological parameters and histopathological findings. Sixty (60) Wister rats were divided into four groups with 15 animals in each group. Group (1): normal group. Group (2): rats injected with CCL4 (0.2ml/100gm) s/c twice a week for 9 weeks. Group (3): rats injected with CCL4 then given MSCs (3x106cell) I/v once a week for 4 weeks. Group (4): rats injected with CCL4 then given levamisole (2.5mg/kg) three successive days for 4 weeks and stem cells (MSCs) (3x106cell) I/v once a week for 4 weeks. Results showed that injection of CCL4 lead to significant increase in activity of hepatic enzymes (AST, ALT, ALP) and Bilirubin level while it decreased serum total protein, albumin, IL- 6, Phagocytic index, Phagocytic %, IgG and IgM. While after treatment with stem cells only or stem cells and levamisole lead to significant decrease in activity of hepatic enzymes (AST, ALT, ALP) and Bilirubin level while it increased serum total protein, albumin, IL- 6, Phagocytic index, Phagocytic %, IgG and IgM.