Abstract of research

Baby Grivet Monkey Kidney (BGM) a new cell line for adaptation of fowl pox virus

El-Bagoury, G.F.1; El-Nahas, E.M.1; Olfat A. Nakhla2 and Nesma M. Hassan2

Abstract

The need to economic, sensitive and easily maintained cell cultures use for massive production of fowl pox vaccine was necessary. We adapted Fowl pox virus (FPV) Baudette strain on Primary chicken embryo fibroblast (CEF), Baby Grivet Monkey Kidney cell line (BGM) and African green monkey kidney (Vero) cell lines. The highest virus titers were 106.5 after the 9th passage in CEF and 106.2 after the 12th passage on BGM while Vero cells were abortive to the virus. BGM cells were selected for study cytopathic effect (CPE) and growth kinetic as it easily maintained than primary CEF cells. The characteristic CPE were rounding till 6th passage with intracytoplasmic inclusion by the 7th passage and syncytium formation appeared from the 9th passage till the 13th passage. The virus was highly cell associated for the first 84h post inoculation(PI) with a titer of 104.5 then a maximum titer was 106.2 in cell free portion after 120h PI. In conclusion, BGM were considered a new susceptible cell for growth of FPV with best harvesting time 120h PI to obtain a maximum titer for subsequent vaccine production.