Abstract of research

Biochemical effect of Moringa peregrina Seeds on experimentally leukcytopenia in Rats

1Abdel-Maksoud, H.A., 2Mohammed, A. Hussein, 1Afaf, D., Abd El Mageed and 1Wafaa Abd El Zaher Taha

Abstract

The present study was to evaluate anti-leukopenia and antioxidant effects of Moringa peregrina seeds oil at dose of 381 mg/kg in 400mg/kg.b.w Benzene and/or 20 mg/kg.b.w 5-Flourourasil induced leukopenia rats 2 weeks after induction of leukopenia in rats, Moringa peregrina seeds oil at dose of 381 mg/kg was administrated for 30 consecutive days. On the 31th day, the rats were sacrificed for the estimation of Hemoglobin (Hb%), Total Leucocyte Count (WBC) and Platelet Count (PLT) as well as biochemical parameters; Alanine Transaminase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP), Albumin(Alb) , Total Protein, Lactate Dehydrogenase (LDH), Creatine Kinase (C.K), Urea , Creatinine ,Reduced Glutathione (GSH), Superoxide Dismutase (SOD), Catalase (CAT), Total Cholesterol (T.Ch), Triglycerides (TG), P53 Gene Expression, Nitric Oxide (NO) , Thiobarbituric Acid Reactive Substances (TBARs) and Tumor Necroses Factor-α (TNF-α). The results of this study also showed that administration of Moringa peregrina seeds oil at dose of 381 mg/kg.b.w to leukopenia induced rats demonstrated a significant (P<0.01) increase in Hb%, WBCs and PLT as well as a significant (P<0.01) improvement in biochemical parameters and life span as compared to the benzene and /or 5-Flourourasil control rats. The histological examinations of this study revealed lung damage and degeneration in the lung of benzene and /or 5-Flourourasil treated rats. Also, lung of Moringa peregrina seeds oil at 381mg/kg rats showed significant improvement and protection against benzene and /or 5-Flourourasil harmful effect. On the other hand, the results clearly suggest that the oxidative stress of benzene higher than 5-Flourourasil