Abstract of research

TRIALS OF PREPARATION AND EVALUATION OF CORYNEBACTERIUMPSEUDOTUBERCULOSIS VACCINES IN SHEEP

Ebeid, M.H.a; Moustafa, A.M.a; Hamoda, F.K. a, Abd el-Azeem, K.A.b khodeir, M.Hb,Bakr, A.A.b, Nabila A. M. Ghazyb

Abstract

Different formulae of oil adjuvant vaccine were prepared from killed local isolated Corynebacterium pseudotuberculosis field isolate and their potency was evaluated in Guinea-pigs and sheep. The cellular and humeral immune response of vaccinated sheep and Guinea pigs were evaluated. The results showed that bacterin and PLD toxoid adjuvanted Montanide oil 206 Corynebacterium pseudotuberculosis is the most suitable vaccine. BCG could be helpful when used before vaccination of sheep with 50 ?g PLD toxoid and 20 mg formalized bacterin adjuvanted Montanide oil to improve the levels of immune response of sheep. The results of postmortem examination of vaccinated and challenged Guinea pigs showed that the mean number of abscesses in skeletal muscles, lymph nodes and internal organs varied to lesser extent between groups vaccinated and control group. All sheep were slaughtered after 150 days and the post mortem examination showed that the different vaccinated sheep groups revealed protection percentages against experimental infection of 71.2-78% while the infection in non-vaccinated sheep was 91%. This results indicated that the prepared vaccine formulae are safe and potent to protect sheep.