Abstract of research

BIOCHEMICAL AND CYTOLOGICAL ALTERATIONS OF PERITONEAL FLUID AFTER INTRAPERIOTENEAL INJECTION OF THIOGLYCOLATTE AND ESHERICHIA COLI IN RATS

Rania M.E.Bakr.1, Farid A.S.1, Abd-Allah O.A.2 and Fararh K.M.1*

Abstract

The differential diagnosis of peritoneal fluid alterations due to pathological conditions is a common clinical problem. However, the capability to distinguish Infectious from noninfectious causes of peritoneal fluid alterations using available biochemical techniques would obviate many expensive and time-consuming diagnostic studies on patients presenting with peritoneal fluid changes of unknown etiology. For demonstrating the effects of chemical and bacterial peritonitis on peritoneal fluid analysis, thioglycolatte medium (TG) and Escherichia coli (E. coli) were used for induction of chemical and bacterial peritonitis, respectively. 90 male rats were divided into 5 groups, each group contains 18 rats. One group used as control, two groups were injected with 3% TG (1or 2 ml/200 gm b.wt.) intraperitonealy and other two groups were injected with E. coli (0.5 or 1×108 CFU/mL) intraperitonealy. Collection of peritoneal fluid samples were done at 1 day, 1 week and 2 weeks after induction of peritonitis. Results of both models showed significant changes in peritoneal fluid analysis. Both modelsshowed significant increases in total protein concentration, specific gravity and cholesterol levels all over the experimental period. Chemical peritonitis model showed significant elevation in triglycerides level at 1 day after induction of peritonitis. While bacterial peritonitis model showed non-significant changes in triglycerides level at all times after induction of peritonitis. Regarding to cytological examination of peritoneal fluid, both modelsshowed significant increases intotal nucleated cell count (TNCC), red blood cells (RBCS), monocytes, neutrophils and mesothelial cell counts at all times after induction of peritonitis. Chemical peritonitis model showed significant increases mainly in mesothelial cells compared to bacterial peritonitis model. On the other hand, bacterial peritonitis model showed significant increases mainly in neutrophils compared to chemical peritonitis. Both models showed significant increases in lymphocytes count at 1 day after induction of peritonitis compared to the control group. Therefore, we concluded that peritoneal fluid could be used for differential diagnosis of peritonitis.