ASSESSMENT OF GENETIC VARIABILITY AMONG FAYOUMI, RHODE ISLAND RED AND THEIR CROSSES USING RAPD-PCR

Samar, H. Baloza1, S. A. Hemeda2, G. A. Sosa3 and Asmaa, Abo Shady4

Abstract

This study was carried out to evaluate the genetic diversity and phylogenetic relationships using randomly amplified polymorphic DNA (RAPD) in four chicken populations; Fayoumi × Fayoumi (FF), Fayoumi × Rhode Island Red (FR), Rhode Island Red× Fayoumi (RF) and Rhode Island Red× Rhode Island Red (RR). Fifteen decamer (RAPD) primers were screened on pooled genomic DNA of four chicken populations produced a total of 154 bands. The number of polymorphic bands was 55.0 with an average of 3.66 per primer. The level of polymorphism among the four chicken genotypes was 36.34%. Genetic similarity between crossbred populations (FR and RF) was (80.28%) while, the similarity between the purebred populations (FF and RR) was (76%). RAPD data showed a clear distinction of Fayoumi from RIR.

Key words

Genetic diversity, Phylogenetic, Fayoumi, RAPD