Abstract of research

BIOCHEMICAL EFFECTS OF ANTI PROTOZOA ON GASTROINTESTINAL TRACT ENZYMES AND RELATED HORMONES IN RABBITS

Afaf D. Abdel-Maged1, Nagwa E. Ahmed2, Ramadan, M.Y.2, M.A. Elashrey1

Abstract

This study aimed to evaluate the biochemical effects of some anti protozoa agents as propolis and toltrazuril in the treatment of coccidiosis disease and their effects on certain gastrointestinal enzymes and related hormones in rabbits. The rabbits were divided into four equal groups each of six rabbits, group 1 control non infected group. How ever, group 2, 3, 4 were infected with 40,000 sporulated oocysts of Eimeria for each rabbit. Group 2 infected non-treated all over the experimental period. Group 3 (propolis treated group) rabbits treated orally with propolis at a dose of 200 mg/kg body weight for 2 weeks after two weeks of infection. Group 4 (toltrazuril treated group) rabbits treated orally with toltrazuril at a dose of 20 mg/kg body weight for 2 weeks after two weeks of infection. Blood samples were collected before, during and after treatment at 2, 4, 6 weeks post infection for biochemical deterimination. The obtained results revealed that, administration of sporulated oocyst of Eimeria species to normal rabbits exhibited marked decrease in amylase, lipase, gastrin, catalase and GST, with an increase in ALT, AST, GGT, ALP and L-MDA compared to control group. In toltrazuril treated group these parameters remains changed after two weeks of treatment. While after two weeks post stoppage of treatment all parameters changed towards the normal values in the control group, and rabbits faeces was free from Eimeria oocysts. On the other hand rabbits treated with propolis showed improvement of all parameters and reversed towards normal control value and rabbits faeces were free from Eimeria oocysts, in addition increase of appetite and body weight was also obtained. These results concluded that, propolis is a drug of choice for treatment of rabbit coccidiosis.