Abstract of research

STUDIES ON DIFFERENT ADJUVANTS AND INACTIVATORS FOR RABIES VACCINE

Amani A. Abassa, El-Bagory, G.F.b and Khodeir, M.H.a

Abstract

This work aims to determine the best inactivator which provides complete inactivation of cell culture adapted rabies virus. The use of 3 concentrations of ßPL induced complete inactivation of rabies virus in final concentration ranged from 1:5000, 1:10000 and 1: 20000 at 4; 6 and 7 hours respectively. The rate of virus inactivation and complete absence of residual virus were confirmed by inoculation of BHK cell culture and weaned mice by intra cerebral route. On the other side, binary ethylenemine (BEI) was used for rabies virus inactivation by cyclization of 2-Bromoethylamine hydrobromide in alkaline solution (sodium hydroxide). Different molarities (0.001, 0.002 and 0.003) of BEI completely inactivated rabies cell culture virus after 18, 12 and 6 hours respectively and these results were confirmed by inoculation of BHK cell culture and mice observed for 15 days post inoculation to detect any signs of rabies. These findings revealed that the used concentration of ßPL and molarities of BEI are the effective factors that determine the time within which complete inactivation of cell culture rabies vaccine could be obtained.