Abstract of research

ENDOTHELIAL DYSFUNCTION IN HYPERCHOLESTEROLEMIC RATS EFFECT OF BEZAFIBRATE ON ENDOTHELIUM–DERIVED SUBSTANCES.

Omayma A Ragab Abozaid, Mohamed R.R.H., and Aml, H.M.

Abstract

The present study was done on sixty male albino rats. They were randomly divided into four groups: the first group (n=10) was fed a normal diet (control). The second group (n=10) fed on normal diet and bezafibrate at a dose of 100 mg/kg B. wt. /day. The third group (n=10) was fed normal diet enriched with 1% cholesterol and 5% coconut oil (cholesterol-fed group). The fourth group (n=30) was fed same as the cholesterol-fed group but supplemented with bezafibrate at concentration 50, 100, 200 mg/kg B. wt. /day. Feeding was continued daily for 8 weeks. Biochemical investigation involved the measurement of total cholesterol, triacylglecerol, high density lipoprotein (HDL), low density lipoprotein (LDL), apolipoprotein B, endothelin-1, histamine, nitric oxide (NO), IL-6, creatinine and alanine amino transferase (ALT).The results of the present study indicate that the total cholesterol, triacylglecerol, apolipoprotein B, and low density lipoprotein (LDL) concentration were significantly elevated in the cholesterol–fed rats compared with the bezafibrate and control rats. Plasma endothelin- 1, histamine and IL-6 concentration were significantly elevated in the cholesterol-fed rats compared with the bezafibrate and control rats. Plasma nitric oxide and high density lipoprotein (HDL) concentration was significantly lowered in the cholesterol fed rats compared to those of bezafibrate treated rats.