Abstract of research

ANTI-INFLAMMATORY AND ANTI-OXIDANT EFFECT OF RUTIN ON 2, 4, 6- TRINITROBENZENE SULFONIC ACID INDUCED ULCERATIVE COLITIS IN RATS.

Samy Ali Hussein; Omayma A.R. Abou Zaid; Abdel-Maksoud, H.A. and Khadija, A.A. Akasha

Abstract

In the present study, the potential protective and therapeutic effect of rutin (RUT) administration on serum nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), colon tissue lipid peroxidation, antioxidant enzymes, reduced glutathione (GSH), and myeloperoxidase (MPO) in trinitrobenzene sulfonic acid (TNBS)-induced ulcerative colitis in rats have been evaluated. Fourty male albino rats were divided into four equal groups of 10 rats each. Group Ι :( Control group): received no drugs. Group Π :( ulcerative colitis -induced group): Administered single intra-colonially dose of 150 mg/kg of TNBS for ulcerative colitis induction. Group III :( ulcerative colitis + RUT protected group): received RUT (200 mg/kg body weight/day) orally for 21 days prior TNBS administration for ulcerative colitis induction. Group IV :( ulcerative colitis + RUT treated group): treated with RUT as in group III for 21 days after ulcerative colitis induction. Blood samples and colon tissue were collected at the 22th day from the onset of RUT administration. The obtained results showed that, TNBS-induced ulcerative colitis caused significant decreased in serum NO level and Glutathione peroxidase (GPx), Superoxide dismutase (SOD), Glutathione –S- transferase (GST) and catalase (CAT) activities in colon tissue. On the other hand, a marked increase in colon tissue Glutathione reductase (GR) and MPO activities and GSH and L-Malondialdehyde (L-MAD) concentrations and in serum TNF-α and IL-1β levels were observed in TNBS induced colitis in rats. Rutin was able to mitigate colon mucosa damage induced by TNBS through increasing of NO, GPX, SOD, CAT, and GST in addition to decreasing L-MDA, TNF-α, IL-1β and MPO activity in colon tissue. These results suggest that, rutin may be effective in enhances the healing of ulcerative colitis by its radical scavenging and anti-inflammatory effect, inhibited neutrophil accumulation, and regenerating endogenous antioxidant mechanisms.