Abstract of research

Detection of FMD virus infection in non-vaccinated and vaccinated cattle and buffaloes in Egyptian governorates during 2016

Gabr F. El-Bagoury 1 , Ehab M. El Nahas 1 , Dina A. Abd-Elwahed 2 , Sayed A.H Salem 2

Abstract

In this study, 2299 serum samples representing 1745 cattle (453 non-vaccinated and 1292 vaccinated) and 554 buffaloes (131 non vaccinated and 423 vaccinated) were collected from 24 different Egyptian governorates during the year 2016. These samples were adopted for detection of FMD virus infection using commercial ELISA kit based on 3ABC nonstructural proteins that differentiate infected from vaccinated animal. Also direct FMD virus serotype detection from 30 vesicular samples, twenty eight from suspected cattle and two from suspected buffalo was carried out using Sandwich typing ELISA. Results showed that, 3ABC ELISA kit were positive 26.04 %( 118/453) of unvaccinated cattle, 39.69 % (52/131) of unvaccinated buffaloes, 23.68% (306/1292) of vaccinated cattle and 34.75% (147/423) of vaccinated buffalo. From a total of 30 samples submitted for direct detection and serotyping of FMDV by Sandwich typing ELISA, 4 were typed as serotype O and one as serotype A. three of serotype O were detected in cattle and the other one in buffalo while the positive sample for serotype A were detected in cattle. In conclusion, FMD virus is still endemic in Egypt despite control program to eradicate and serotype O was the most prevalent serotype in cattle and buffaloes.