Recent isolation and identification of a lumpy skin disease virus from Qaluobia Province, Egypt 2016

Gabr F. El-Bagoury 1 , Ehab M. El Nahas 1 , Nashwa M. Hassan 2 , Mohammed G. El-Hammady2

Abstract

Cases of skin nodules eruptions suspected for lumpy skin disease virus (LSDV) are becoming more frequent in Qaluobia Province, Egypt in cattle populations during summer and autumn of 2016. In this study, LSDV associated with the outbreak was isolated from skin nodules of unvaccinated cattle on chorioallantoic membrane (CAM) of specific pathogen free embryonated chicken eggs (SPF-ECEs) and Madin derby bovine kidney (MDBK) cell line. The isolate induced pock lesion on CAM and rounding, aggregation and detachment of MDBK cells. Identification of LSDV isolate was confirmed by neutralization test and polymerase chain reaction (PCR) targeting LSDV fusion protein encoding gene. In conclusion, LSDV is still circulated in Egypt and further molecular and biological characterization is required.

Key words

LSDV; cattle; chick embryos; Neutralization, PCR; Egypt.