Abstract of research

Bacteriological and Molecular Studies on some Bacteria Isolated From Mastitic Cattle and Humans Contact

Fawzia A. 3 Khalid I. El-ekhnawey and Hofy, 2 Fatma, I. El- Tawab, 1 Ashraf A. Abd EL1 El-Shenawey

Abstract

This study was done on a total of 92 mastitic milk samples (50 clinical, 42 sub-clinical) and 40 hand swabs from contact humans were collected from different dairy farms at Gharbia governorate. The collected samples were examined bacteriologically to isolate mastitis pathogens (Staph. aureus, Strept. agalactiae, S. dysgalactiae and Strept. uberis). From clinical mastitic samples, six isolates were S. aureus ( 12%) and one isolate was (2%) S. dysgalactiae. Among sub clinical mastitic milk samples two isolates were S. aureus (4.6%) and one isolate (2.3%) S. agalactiae. While S. uberis were not detected. From contact human hand swabs both S. aureus and Streptococcus species were not detected. Antibiotic sensitivity test revealed that all bacterial isolates were highly sensitive to enerofloxacin, ciprofloxacin, sulpha |trimethoprim and gentamicin respectively, while all isolates were resistant to penicillin followed by amoxicillin/Clavulanic acid. Two isolates of S. aureus were screened for detection of enterotoxin genes (Sea, Seb, Sec, Sed and See) by multiplex PCR. Only Sed gene was detected in one isolate. Cfb gene (CAMP factor) and hyl (hyluronidase) gene were detected in S. agalactiae. mig (surface-expressed mig protein) gene was detected in S. dysgalactiae.