Trial for Preparation of Combined Equine Influenza virus, Equine Herpesvirus-1 and Tetanus Toxoid Vaccine

Mohamed bayoumi1 , Abdelmoneim M. Moustafa2 , Nashwa Madkour 1 , Abdelfattah Selim2 , Wafaa Abdelazez1

Abstract

Vaccination remains an important strategy to fight Equine influenza (EI), Equine herpesvirus-1 (EHV1) and tetanus infection, in combination with management measures. A combined inactivated EIV, EHV-1 and Tetanus toxoid (TT) vaccine adjuvanted with saponin and Alhydrogel was prepared and tested in four groups of guinea pigs in comparison with each vaccine separately beside another group as control. The humoral immune response in vaccinated guinea pigs against EI, EHV-1 and tetanus was evaluated by Haemagglutination Inhibition (HI) antibody titers, Enzyme linked immunosorbent assay (ELISA) titers, and toxin neutralizing (TN) antibody titers respectively. All vaccines proved to be safe and potent in guinea pigs. Also, the obtained antibodies titers with combined vaccine was nearly similar to titer obtained with separated vaccine of each target agent in vaccinated guinea pigs. The evaluation of keeping quality of the prepared combined inactivated vaccine showed that it is was stable at 4C up to 12 months and for 3 months at room temperature.

Key words

Equine influenza, herpesvirus-1, Tetanus toxoid, saponin, Alhydrogel vaccine, Potency