Abstract of research

PARENTAGE TESTING OF ARABIAN HORSE IN EGYPT USING MICROSATELLITE DNA TYPING

Mary A.N. Sargious1; Bakry H.2; El-Shawarby, R.M.2, Hanaa A. Ahmed1.

Abstract

This study was carried to establish a parentage testing system for the Arabian horses in Egypt. Thirtyeight Arabian horse samples including seventeen foals, five sires and sixteen dams from El Zahraa stud were genotyped by using seventeen microsatellite markers recommended by the International Society for Animal Genetics (ISAG). The number of alleles varied from 4 (ASB23, HMS2) to 13 (VHL20). The observed heterozygosity and expected heterozygosity ranged from 0.27 (HMS3) to 0.816 (HTG10) and from 0.474 (ASB23) to 0.841 (ASB17) respectively. PIC value ranged from 0.423 (ASB23) to 0.812 (ASB17) with average 0.650. There was 100 % matching between pedigree and DNA typing parentage for the 17 examined foals. These results suggested that the DNA typing method had high potential for parentage verification and individual identification of the Arabian horses in Egypt.