Abstract of research

BIOCHEMICAL EVALUATION OF CHEMOPREVENTION EFFICACY OF N-ACETYL CYSTEINE, ZINC OXIDE AND HIGH PH ASCORBATE ON MAMMARY GLAND CARCINOMA USING NANOTECHNOLOGY.

Omayma A.R. Abou Zaid 1; Abdelfattah Badawi.2 and Sameh, A. Elsayes 1

Abstract

The present study was designed to evaluate the prevention efficacy of the novel prepared compound, high pH sodium ascorbate combined with N-acetyl cysteine / Zinc oxide nanocomposite in vivo on the incidence of 7, 12- dimethylbenz[a]anthracene-induced mammary tumor in female rats. The prevention efficacy of such treatment was evaluated by measuring the activity of caspase-3and caspase-9 in mammary gland homogenate in addition to the activities of the most important free radical scavengers of the antioxidant defense system in breast tissue including; reduced glutathione (GSH), glutathione peroxidase ( GPx ), superoxide dismutase ( SOD ), catalase (CAT), as well as malondialdehyde content (MAD) which is considered an indicator of lipid peroxidation. The result of the present study revealed that our novel tested compound, regarding their in vivo anti-tumor effect, so that combination between them as it could ameliorate or normalize most of the investigated parameters. In conclusion, these findings suggest that novel synthetic Nano composite and high pH ascorbate may potentially presents new hope for the development of breast cancer prevention, which should attract further scientific and pharmaceutical interest.