Ameliorating role of resveratrol on biochemical changes in experimentallyinduced liver fibrosis in rats

Samy Ali Hussein1*; Samir Abdel Latif Abdel Aal 2; Mohammed Bader Abd El-Aziem3 and Hisham Fathey EL Said 1


Liver fibrosis is the final pathway stage of most chronic liver diseases, and is the main reason for increased mortality in affected patients. Hence, this study was undertaken to evaluate the hepatoprotective effect and the efficacies of resveratrol against thioacetamide (TAA) induced liver fibrosis in rats. The experimental model of liver fibrosis in rats was induced by intraperitoneal (i.p) injection of TAA at a dose (200mg/Kg b.wt / thrice / week) for 6 weeks. A total of fifty five male rats were used in this study. All rats were divided into four groups. Group I: (Control normal group) rats received no drugs. Group II: (TAA- group). Group III: (TAA + resveratrol protected group) rats injected with TAA and administered resveratrol (0.5 mg/Kg body weight / day, intraperitoneally) from the 7th week to 12th week. Group IV: (TAA + resveratrol treated group) rats firstly injected with TAA from the 1st week to 6th week and treated daily with resveratrol (0.5 mg/Kg body weight, intraperitoneally) from the 7th week to 12th week. Blood samples and liver tissue were taken and processed directly for some biochemical parameters determination. The obtained results revealed that, a significant increase in serum ALT, AST and ALP activities, IL-6, TGF-β1 and liver L-MDA concentrations were observed in TAA injected rats. However, administration of resveratrol to TAA induced liver fibrosis in rats exhibited a significant decreased in all mentioned parameters and attenuated the increased L-MDA concentration in liver tissues. On the other hand, a significant decrease in liver antioxidant enzymes (SOD and CAT) activities were observed in TAA injected rats when compared with control normal group. Meanwhile, resveratrol administration resulted in a significant increase in liver SOD and CAT activities. It could be concluded that, inhibition of lipid peroxidation, inflammation and oxidative stress and enhanced antioxidant enzymatic status in rats liver by resveratrol suggest the potential efficiency of resveratrol as a natural hepatoprotective and anti-inflammatory agent in treatment of liver fibrosis.

Key words

Thioacetamide (TAA), resveratrol (RSV), oxidative stress, inflammatory markers, liver fibrosis.

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