A real-time PCR, an accurate method for detection of salmonella in chickens

Ashraf A. Abd El-Tawab1,Wafaa M. Hassan2, Fawzi R. El-Saidi3, DaliaM. Ayoup4


A total of 100 sample were obtained from different slaughter houses located in cairo, Living chickens(20 cloacal swabs ), Viscera (20 cecum and 20 internal organs ), 20 swabs from Chilling water and 20 swabs from final product, The samples were examined bacteriologically and serologically for Salmonella. where was 4% from total samples . The serotyping was two S.kentuky , one S.Tamilandu and one S.Anatum. The four isolated salmonella serovars were tested for antibiotic sensitivity and was found one isolate of Salmonella Kentuky was sensitive to all antibiotics while the other one was resistant to all antibiotics except (neomycin) was intermediate , S.Tamilandu was sensitive to all antibiotics except ( neomycin and colistin sulphate ) was resistant , S. Anatum was sensitive to neomycin, norfloxacin and ciprofloxacin , while resistant to nalidixic acid , cefatoxine ,ceftriaxon , gentamycin , amoxicillin and colistin sulphate and intermediate to ampicillin.Real time PCR was used for detection of salmonella, the positive Salmonella reference strain was ATCC 14028 and negative control was E.coli ATCC 25922. The number of positive Salmonella samples which was detected by RT-PCR was six samples while was four samples was No. of positive samples detected by stander method

Key words

Salmonella, Real-time PCR, Chickens, slaughter houses

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