Abstract of research

Protective role of new nanocomposite natural product (basic nano-curcumin) against breast carcinoma

Omayma A.R. Abou Zaid1, Fatma A. Ahmed2, Abdel Fatah M. Badwi3, Naglaa M.H. Ibrahim2

Abstract

Present study focused on evaluation of the effect of national nancomposite product on serum biochemical profile and programmed cell death (apoptosis) by using Annexin V-FITC and PI test of breast carcinoma female rats. Basic nanocurcumin was tested for its protective effect on breast cancer female rats (n= 30) divided in two main groups (NTBR) and (TBR) which in turn classified into (TBR- Control Positive and TBR- basic nano- curcumin protective groups). Basic nano-curcumin was formulated and tested for both IC50 (IC50 = 1.0 μg) and LD5o (35 mg/kg by wt.) by using in vitro and in vivo techniques, respectively. Breast cells apoptosis was determined by Annexin V-FITC, which is a fluorescent probe binds to phosphotidylserine (PS) in a Ca 2+ dependent pathway. Breast Cells were stained with Annexin V and PI. The apoptotic cells [Annexin (+) and PI (-)] were differentiated from necrotic cells [Annexin (-) and PI (+)] using flow cytometry technique. The anti-proliferative effect of basic nano-curcumin appeared much earlier due to its nano form. Also, Liver [ALT (40U/l), AST (85 U/l)] and kidney [BUN (19 mg/dl), Creatinine (0.5 mg/dl)] functions were decreased within normal ranges.