Abstract of research

BIOCHEMICAL EFFECTS OF PROPOLIS AND BEE POLLEN IN EXPERIMENTALLY – INDUCED HYPERAMMONEMIA IN RATS

1Omnia M. A, 2Nabila M.A, 3Nadia R.R.

Abstract

The main objective of this is study to investigate the biochemical effects of propolis and pollen grains as natural antioxidants on thioacetamide (TAA) at a dose of (150 mg/kg/bw single dose intraperitonelly (i.p. ) induced hyperammonemia in rats. One hundred male albino rats were divided into 5 group (20 each). Group (1) act as a control group (2) injected with TAA at dose 150 mg/kg/bw i.p.) act as control hyperammonemia group (3) injected with TAA at a dose 150 mg/kg/bw i.p.) and treated with propolis at a dose 300 mg/kg/bw, group (4) injected with TAA at a dose 150 mg/kg/bw i.p.) and treated with bee pollen grains at a dose 25 g/kg/bw and group (5) injected with TAA at a dose 150 mg/kg/bw i.p.) and treated with both propolis and pollen grains with the same dose for two months. Serum was separated twice after 30 and 60 days of treatment all serum was collected for estimation of Aspartate aminotransferase (SGOT), Alanine aminotransferase (SGPT), Alkaline phosphatase (ALP), Gamma glutamyl transferase (GGT), albumin, total protein, urea, creatinine, uric acid, gamm amino butyric acid ( GABA), cholinesterase , N-acetyl glutamate synthase (NAGS), nitric oxide (NO) and plasma ammonia in control and propolis and pollen treated rats against TAA- induced hyperammonemia in rats. liver, brain and kidney were collected for determination superoxide dismutase (SOD), catalase ( CAT) and Lmalonialdehyde (L-MDA), results revealed a significant decreased in serum SGOT, SGPT, ALP, GGT, urea, creatinine, uric acid, gamm amino butyric acid ( GABA), cholinesterase , nitric oxide (NO), plasma ammonia, L-MDA in tissues and also marked significantly increased in albumin, total protein, N-acetyl glutamate synthase (NAGS) and CAT, SOD in liver , kidney and brain tissues. The behavioral biochemical results indicated the effect of pollen grains and propolis against TAA- induced hyperammonemia in rats.