Abstract of research

Resveratrol ameliorates the biochemical changes in fructose induced insulin resistance in rats

Samy A. Hussein, Shawky A. A. Mostafa, Mohammed B. Abd El-Aziem, Aya A. Abo Alhamd

Abstract

There is evidence that high-fructose diet induces insulin resistance, alterations in lipid metabolism, and oxidative stress in rat tissues. The purpose of this study was to evaluate the effect of resveratrol (RSV) administration on biochemical parameters, cytokines, inflammation, oxidative stress, antioxidant status, and lipid metabolism in male rats fed with high fructose diet. Insulin resistance was induced by feeding high fructose diet (60 g/100 g). Forty-five male albino rats were divided into three groups containing 15 rats each. Group Î™:(Control group) rats received the control diets. Group II (fructose-fed group) rats received fructose- enriched diet (60 g /100g). Group III: (fructose + RSV group) rats received high fructose diet and administered RSV (0.5 mg/Kg body weight /day, intraperitoneally). After 6 and 8weeks of treatment blood samples and liver tissue were collected for determination of serum glucose, insulin, insulin resistance, total cholesterol, triacylglycerols, Adiponectin, tumor necrosis factor- Î± (TNF- Î±) and interleukin-6 (IL-6) in addition to antioxidant enzyme (CAT), non-enzymatic antioxidant (GSH), L-Malondialdehyde (L-MDA) and Nuclear Factor kappa B (NF-ÐºB) in liver tissues were also determined. The obtained results revealed that, high fructose diet induce a significant increase in serum glucose, insulin, insulin resistance, cholesterol, triacylglycerols, TNF-Î± and IL-6 concentrations and decrease in serum adiponectin, with marked reduction in CAT and GSH concentrations in liver tissues and marked increase in L-MDA and NF-kBin liver tissues. Resveratrol treatment to high fructose fed rats reduced the effects of fructose and associated with significant normalization of all serum parameters level and was able to improve dyslipidemia, inflammation and insulin resistance, attenuated the increased L-MDA, enhanced antioxidant status in liver tissues. These results suggest that, Resveratrol is effective in improving the high fructose induced oxidative stress, inflammation and insulin resistance in male rats. Also, the administration of Resveratrol to rats fed a high fructose diet prevents the development of oxidative stress and its associated complications include hyperglycemia, hyperinsulinemia and dyslipidemia.