Abstract of research

BIOCHEMICAL EFFECT OF SOME HERBAL MEDICINE IN EXPERIMENTALLY INDUCED POLY CYSTIC OVARIES

Abd El-Maksoud H.A.a,*, Hassanin M.R.a, Eman, A. Gabra

Abstract

In the present study, the effect of herbal medicine on serum glucose, lipid profile, progestrone, LH and FSH hormones in experimentally induced poly cystic ovaries in female rats have been evaluated. This study was carried out in vitro on 84 female rats (21-days old). The rats were divided into main two groups. Group Ι: (Control group): include twelve female rats and kept as control, and Group Π: (induced PCOS group): include seventy two female rats were injected subcutaneously (S/C) by testosterone propionate by a dose of 1 mg/100 gm body weight daily and still for 35 days. The later was divided into six sub groups each contain twelve females rats. Sub group 1: (control PCOS): did not receive any herbal medication and sub group 2 :( induced PCOS Salvia officinalis treated group). Sub group 3: (induced PCOS Origanum vulgare treated group) and sub group 4: (induced PCOS Adiantum capillus–veneris treated group). Sub group 5: (induced PCOS Equisetum arvense treated group) and sub group 6: (induced PCOS treated group with mix of the mentioned herbs). The study also carried out in vivo on ten women with PCOS and ten healthy women kept as control. Blood samples for serum separation were collected from all animals groups three times after 10, 20 and 30 days from the onset of herbal treatment begin after induction of the syndrome, and from women at the 2nd day of menstruation. All sera were processed directly for determination of glucose, total cholesterol, triacylglycerols, HDL–C, LDL-C, VLDL-C, progestrone, LH and FSH hormones. The obtained results revealed that there was a similarity in the biochemical changes of both PCOS women and experimentally induced PCOS female rats, which reported as a significant increase in the mean values of serum glucose, total cholesterol, triacylglycerols, LDL-C, VLDL-C, HDL-C and LH hormone while a significant decrease in the mean values of serum HDL-C and progestrone concentration was observed. From the obtained results of the present study it could be concluded that administration of S. officinalis, O. vulgare and the herbal mix in women with PCOS are very essential and should be used with therapeutic dose level which may improve the progression of the disease and attenuate the undesirable effect of dangerous metabolic and hormonal changes in women with PCOS.