Abstract of research

Preparation and field evaluation of live attenuated sheep pox vaccine for protection of calves against lumpy skin disease

Heba A. Khafagy , Mohamed G. Abdelwahab , Abdelmoneim M. Mustafa , Mohamed A. Saad

Abstract

In this research Romanian Sheep pox virus was identified and confirmed by using PCR, Live attenuated Romanian Sheep pox vaccine (RSPV) was prepared and its titer on VERO cell was log 102.5TCID50/dose. It was sterile, safe and potent. We used eight susceptible calves 6-8 months old, five calves vaccinated with 0.5 ml of prepared RSP vaccine intra dermally (I/D) in tail folds while three calves kept as control. Evaluation of both acquired humoral and cellular immunity by using lymphocyte proliferation assay, SNT, ELISA and Interferon Gamma Bioassay (IFN-áµ§). The result showed that lymphocyte proliferation began to increase till reach to its peak (1.629) at 10th day then decrease after that, While Interferon gamma (IFN-áµ§) detected in 1st day till 7th day post vaccination then decreases after that. Results of SNT and ELISA revealed that Protective serum neutralizing antibody titer started at three weeks (1.5), (1.09) post vaccination then reach to its peak at 12th weeks (2.5), (1.85) respectively and persisted till 28 weeks. From this study we found that live attenuated RSP vaccine a good immunogenic response where it was induced a high level of antibody titer with prolonged duration of immunity and higher lymphocyte and interferon gamma levels, So It considered good vaccine to control Lumpy Skin Disease (LSD).