Molecular detection of some virulence genes of S. aureus isolated from mastitic Cows by PCR.

Ashraf A. Abdel-Tawab1, Ahmed A.A. Maarouf 2, Fatma I. El-Hofy1, Sameh A.H. Abbas3

Abstract

In this study a total of 1200 quarter milk samples were selected from 300 cows at different localities of El-Kaliobia Governorate were examined bacteriologically and revealed that 57 S.aureus isolated from both clinical and subclinical mastitis with incidence of 45.6% of total isolates. Molecular typing of S. aureus by PCR revealed that all isolates (100%) (12 random S.aureus isolates) were positive for the 16 SrRNA genes of S. aureus. Genotyping of virulence genes encoding factors of S. aureus revealed that two isolates were positive for (tst-1) gene (16.7%), three isolates positive for (etb) 25%, nine isolates positive for each of (icaD and femA) genes (75%), seven isolates positive for (hlg) (58.3%), eight isolates positive for (nuc) (66.7%).

Key words

Mastitis, S. aureus, PCR, virulence genes.