ISOLATION AND GENOTYPING OF BOVINE VIRAL DIARRHEA VIRUS: FIELD ISOLATES FROM INFECTED CATTLE IN KALUOBIA DURING 2011.

G.F. El-Bagourya, E.M. El-Nahasa, K.A. Khadrb, and Nawal M.Alic

Abstract

In the present study, we report the isolation of bovine viral diarrhea virus (BVDV) field strains from clinically infected cattle. Sixty samples including 54 buffy coat and 6 nasal swabs were collected from clinically infected cattle in Kaluobia during the year 2011. Screening of samples by Antigen capture ELISA kites revealed ten positive samples for the presence of BVDV antigen. In a trail for isolation of BVDV, 8 buffy coat and 2 nasal swabs were inoculated in MDBK cells. Eight selected samples that induced a clear cytopatheic effect on inoculated cells and 2 non-cytopathic were subjected for further identification and genotyping using nested PCR assay. The results revealed that the isolated BVDV field strains from Kaluobia 2011 were of BVDV type 1.

Key words

BVDV, ELISA, MDBK, Nested PCR Assay