Molecular studies on antimicrobial resistance genes in salmonella isolated from poultry flocks in Egypt

Ashraf A. Abd El- Tawab a, Ahmed M. Ammar b, Soad A. Nasef c, Fatma I. El- Hofya and Nehal M. Nabil d.

Abstract


This study was aimed to investigate the wide spread resistance to different antimicrobial groups in between different Salmonellae isolated from poultry flocks in Egypt. A total of 63 Salmonella isolates (19 from chickens, 3 from ducks, 2 from turkeys, 2 from quails and 2 from pigeons) were examined in this study for their wide spread resistance against different antimicrobial agents. The antimicrobial susceptibility was applied on all isolates then PCR was applied for all resistant isolates to detect the resistance genes of different antimicrobial agents (qepA, qnrS, aac (6') - ib-cr) for quinolone resistant isolates, bla Tem for ? lactam resistant isolates, sul1 for Sulfonamide resistant isolates, floR for Florphenicol resistant isolates, aadA2 for Streptomycin resistant isolates and tetA (A) for Oxytetracycline resistant isolates, in addition to gyrase enzyme gene (gyrA). DNA sequencing was done for three selected isolates to detect possible mutations in the quinolone-resistance determining regions of the gyrA gene.

Key words


Salmonella, resistance genes, poultry

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