Abstract of research

EFFECT OF FEEDING PROBIOTIC ON HEMATOLOGICAL, BIOCHEMICAL PROPERTIES AND IMMUNE RESPONSE IN BROILER

Salim, H.A.a, Abd-Allah, O. A.b, and Fararh, K.M.a

Abstract

The research work was conducted on “Hubbard breeds” broilers to evaluate the effect of probiotics on leukocytes, biochemical parameters and immune response. One day old broiler chicks were randomly divided into four groups as follow: control group, probiotic fed group (NPRO), infected group with salmonella typhimurium (INT, non-treated with probiotic) and infected treated group (IPRO). Bacillus subtilis was the main constituent of the Probiotic. Results of probiotic supplementation revealed significant leukocytosis and lymphocytosis, hyperprotinemia, hyperglobinemia, and significant decrease in triglycerides, cholesterol, and glucose without significant change in AST, ALT, uric acid and creatinine. Significant increase in HI titer, phagocytic activity and phagocytic index was observed. Infection with Salmonella typhimurium showed leukocytosis, heterophilia and lymphopenia. hypoproteinemia, hypoalbuminemia, elevation of liver enzymes (AST and ALT), uric acid, and creatinine which indicate damage of liver and kidney . Immunological parameters revealed increase in serum alpha and beta globulins, and significant decrease in Phagocytic activity and Phagocytic index. In IPRO group, Bacillus subtilis decreased elevated liver enzymes, uric acid and creatinine. Probiotic also reduce the percentage of serum triglycerides and total cholesterol. From the results of this study we can concluded that probiotic Samu Biogen (bacillus subtilis) had clear impact in increasing leukocyte and immune response which appeared to reduce the damaging effect of Salmonella typhimurium infection on liver and kidney.