Abstract of research

Comparative evaluation of standard serological tests for diagnosis of ovine brucellosis

Ashraf, A. Abd El Tawwab 1, Fatma I. El Hofy1, Khoudair M.Ramadan.2, Fatma E. Harb.3

Abstract

One hundred blood samples (55 sheep and45 goats) (include 79 females and 21 males)were selected from farms suffer from brucellosis and examined serologically by Buffered Acidified plate Antigen Test (BAPAT), Rose Bengal plate Antigen test (RBPAT) , Tube Agglutination test (TAT), Complement Fixation Test (CFT) and Immunochromatography assay (ICA). The results of BAPAT were (61.81%) and (73.33%), RBPAT& TAT were (61.81%) and (66.66%) and CFT &ICA were (60%) and (66.66%) in sheep and goats respectively for all tests. Moreover, the positive reactors in females among sheep and goats were (79.1%), (76.56%), (81.25%), (80.95%) and (79.36%) using BAPAT, RBPAT, TAT, CFT and ICA respectively. The sensitivity, specificity and agreement of BAPAT with CFT in sheep and goats were (92.06%, 75.67%, 86%) respectively. The sensitivity, specificity and agreement of RBPT with CFT in sheep and goats were (88.88%, 78.37%, 85%) respectively. The sensitivity, specificity and agreement of TAT with CFT in sheep and goats were (84.12%, 70.27%, 79%) respectively. The sensitivity, specificity and agreement of ICA with CFT in sheep and goats were (98.41%, 97.29%, 98%) respectively. The sensitivity, specificity of CFT was 100%. In conclusion the ICA proved to be the most accurate, cheapest, rapid and simplest test for diagnosis of ovine brucellosis.