Assessment of oxytetracycline and ampicillin residues in sheep carcasses

Hemmat M. Al-Gendy1, Faten S. Hasanen1, Amani M. Salem1, Shaimaa M. Nada 2

Abstract


This study was conducted for assessment of oxytetracycline and ampicillin residues in ninety sheep carcasses (meat, liver &kidney 30 of each) collected from two different abattoirs (urban and rural area 45 of each) in Menofia governorate, by using four plate test (FPT) and high performance liquid chromatography (HPLC) technique. The results revealed that the incidence of oxytetracycline and ampicillin residues in the examined samples of sheep meat, liver and kidney were 6.67% , 20% & 20 % and 0% ,13.33% &13.33% with application of FPT and 13.33%, 20% &33.33% and 13.33%, 20% &26.67% by using HPLC from urban area, respectively.While,13.33%,26.67% &33.33% and 6.67%, 20% &20% for those samples meat, liver and kidney with application of FPT and 20%, 33.33% &33.33% and 13.33%, 26.67% &33.33% by using HPLC from rural area, respectively. However, the mean values of oxytetracycline and ampicillin residues (μg/kg) were 109.5±17.3, 584.7±41& 1115.2±98.7 and 17.5±1.1, 50±4.8 &72.3±6.2 for samples in urban area and 178.3±22.8, 697.2±59.1 & 1291.6±117.5 and 28.5±1.9, 57.7±5.2 &85.8±9.4 for those in rural area, respectively. Accurately 0%, 13.33% & 13.33% and 6.67%, 20% & 26.67% of both samples from urban and rural areas were unaccepted based on their contents of oxytetracycline according to FAO/WHO (2004), respectively. In addition, accurately 6.67% &20% and 13.33%& 20% of liver and kidney samples for urban and rural areas were unaccepted, while all meat samples were accepted according to FAO/WHO (2004), respectively. The public health significance of antibiotics residues and the suggestive recommendations to avoid the presence of such residues were discussed in this study.

Key words


Sheep meat, offals, oxytetracycline, ampicillin.

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