Abstract of research

Biochemical role of wheat germ oil on biomarkers of oxidative stress and inflammatory response in a rat model of endotoxemia

Samy Ali Hussein 1; Abdel-Aal, S.A. 2; and Elghwab, A.I.M. 1

Abstract

The main objective of this study was to investigate the protective effect of wheat germ oil on oxidative stress induced by endotoxin in rats. Fourty five male rats divided into three equal groups of (15 each). Group I (control): rats received no drugs. Group II (Endotoxin group): rats injected intraperitoneally (i.p) with a single dose of endotoxin (200 mg/Kg b.wt). Group III (wheat germ oil protected group): rats pretreated with wheat germ oil (270 mg/kg b.wt./day) orally for 21 day then injected with a single dose of endotoxin (200 mg/Kg b.wt). Blood samples for serum separation and liver tissues were collected from all animal groups after 1 and 3 hours from the onset of endotoxin injection. Serum tumor necrosis factor- alpha (TNF-α), interleukin-6 (IL-6), L-Malondialdehyde (L-MDA), Nitric oxide (NO) and sialic acid, in addition to liver tissue superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), myeloperoxidase (MPO), reduced glutathione (GSH),Glutathione S-transferase (GST) and LMalondialdehyde (MDA) were determined. The obtained results revealed that, endotoxemia could potentially increase serum TNF-α, IL-6, L-MDA concentrations and markedly decrease NO and sialic acid levels. Moreover, SOD, CAT and MPO activities and GSH concentration in liver tissues were significantly decreased. Pretreatment with wheat germ oil decreased LPS-triggered pathogenic responses and prevented the increase of TNF-α, IL-6 and L-MDA. Moreover, pretreatment with wheat germ oil in endotoxin injected rats responsible for the protection of membrane polyunsaturated fatty acids against lipid peroxidation by increasing the activity of liver tissue antioxidant enzymes GST, SOD and CAT. From the obtained results it could be concluded that, wheat germ oil protects against lipid peroxidation, oxidative stress and decrease the inflammatory response induced by endotoxin injection in rats.