Abstract of research

DETECTION OF SOME VIRULENCE GENES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RABBITS BY POLYMERASE CHAIN REACTION.

1Ashraf, A. AbdEl-Tawab, 2Ahmed, A. A. Maarouf, 1Fatma, I. El-Hofy and 2Amany, O.S. Khalil

Abstract

The present study was performed on a total of 260 rabbits (48 diseased and 212 freshly dead ones) from rabbit farms at Kaliobia Governorate that inspected for S. aureus. Samples were taken from these rabbits (liver; heart blood; lung; intestine; kidney and spleen from each one) after clinical and postmortem examinationfor bacteriological examination. The results revealed that 314 out of 1560 samples (20.1%) were positive for S. aureus isolation, where 30 isolates (1.9%) from 288 samples of 48 diseased rabbits and 284 isolates (18.2%) from 1272samples of212 freshly dead ones.Moreover, higher rates of isolation of S. aureus from; liver (28.0%); heart blood (23.6%) ; lungs (22.0%) ; intestine (16.9%); kidneys (6.7%) and finally spleen (2. 9%) .Ciprofloxacin, Gentamycin, Norfloxacin and Sulpha trimethoprim were the most proper antibiotics with the highest in vitro efficiency against isolated S. aureus. PCR results showed that spa and clfA virulence genes were detected in 9 studied strains. While hlyA virulence gene was detected in 4 strains, ica A virulence gene was detected in 5 strains and Enterotoxins Sea, sec were detected in 4 out of 5 studied strains. Moreover, leukocidine (pvl) and enterotoxins (seb, sed, see) virulence genes were not detected in all studied strains. In addition, these results conceded the results obtained by dry spot Staphytect plus card test for spa and clfA.