INACTIVATION OF VERO CELL CULTURE ADAPTED CHICKEN ANEMIA VIRUS VACCINE

Samah, S. Abou-Dalal 1, El-Bagoury, G.F 2, Suzan, K.Tolba1, Khodeir, M.H. 1

Abstract

CAV is usually propagated on MDCC cell-culture and because this type of cells are not available in Egypt. We have used Vero cell line instead. CAV was propagated fourteen serial passages in Vero cell culture. The cytopathic effect in cell culture (CPE) was characterized vacuolation of the infected monolayers and subsequent detachment. Vacuolation of the infected monolayers started by the 7th day post infection (DPI) then began to appear more early by the successive passage to reach the 2nd DPI cell. The highest virus titer was 7.6 log10TCID50 /ml in Vero cell culture obtained by the 10th passage. Studying the growth kinetics of CAV in Vero cell culture indicated that the highest total virus yield was 7.6log10TCID50/ml by 72 hours post cell infection. Complete inactivation of CAV was obtained after 72 hours at 37oC using 0.2% formalin. Complete virus inactivation was confirmed by 3 successive passages in Vero cell culture showing absence of CPE and FAT showing negative reaction.

Key words

CAV, Vero, Inactivation, Formalin