Abstract of research

COMPARATIVE STUDY ON LIVE ATTENUATED AND INACTIVATED CHICKEN ANEMIA VIRUS VACCINES

El-Bagoury, G.F1, Samah, S. Abou-Dalal2, Suzan, K.Tolba2, Khodeir, M.H. 2

Abstract

The aim of this research was to compare and evaluate the potency and safety of live and inactivated vaccines. This has been investigated by using 180 specific pathogen-free (SPF) chickens. 100 chickens were given the live vaccine, 50 were given the inactivated vaccine and 30 were used as non-vaccinated control. The antibody titer was measured periodically until 40 weeks post vaccination using ELISA and SNT. The results showed that the live CAV vaccinated birds exhibited detectable levels of specific CAV antibodies by the 1st week recording peak titers by the 8th week post vaccination. In contact non-vaccinated birds attracted the excreted virus from vaccinated chickens and exhibited lower antibody titers. Chickens vaccinated with inactivated CAV vaccine showed detectable specific CAV antibodies by 1st week post vaccination recorded that the peak titer for both groups by the 8th week post vaccination. These titers began to decline by the 32nd week post vaccination. Away control non-vaccinated chickens remain sero-negative in both groups. Evaluation of the hematocrit values in chickens vaccinated with the live CAV vaccine showed decreased levels by the 2ndweek post vaccination then began to return to safe levels started by the 3rd week. On the other side all chicken groups vaccinated with the inactivated CAV vaccine did not show decline in their hematocrit values as well as in contact and away control non-vaccinated chickens confirmed the complete safety of such vaccine. We concluded from this study that the inactivated CAV vaccine was highly immunogenic and safer than the live vaccine.