Abstract of research

BIOCHEMICAL EFFECT OF EMBLICA OFFICINALIS ON EXPERIMENTALLY INDUCED TUMOR IN FEMALE MICE

Ali, H. A. a, El-Senosi, Y. A. a and El-Bialy, M.M.A. a

Abstract

This study was carried out on 150 Australian female mice; 12-14 weeks old and weighted 25-30 gm. Mice were classified into two main large experiments. Experiment A: Non-tumor bearing mice (NTB) Included 60 of animals and divided into three groups each one comprised 20 mice. Group 1: NTBcontrol saline treated. Group 2: NTB-treated with Emblica officinalis orally (500 mg/kg/every alternate day) for 4 weeks. Group 3: NTB-treated with Emblica officinalis orally (1250 mg/kg/ every alternate day) for 4 weeks. . Experiment B: Tumor bearing (TB) mice. Included 90 of animals and divided into three groups each one comprised 30 mice. Group 1: TBM-control saline treated. Group 2: TBM-treated with Emblica officinalis orally (500 mg/kg/every alternate day 24 hours), after inoculation of ascetic fluid for 4weeks. Group 3: TBM-treated with Emblica officinalis orally (1250 mg/kg/ every alternate day) , 24 hours after inoculation of ascetic fluid for 4 weeks. Blood samples were collected from all animals groups after 1, 2 and 3 weeks from treatment. Serum blood were separated and processed directly for (AST and ALT) activities, (bilirubin, and creatinine) concentrations and antioxidant enzymes activities ( catalase ,superoxide dismutase SOD, reduced glutathione GSH). The obtained results revealed significant increases in serum AST, ALT, ALP, bilirubin and creatinine concentrations. On contrary, significant decreases in serum GSH, SOD and catalase activity were observed in tumor bearing mice when compared with control. The results of this study indicated that Emblica officinalis treatment has potential benefits in cancer treatment in female mice.