Abstract of research

PROTECTIVE EFFECT OF VITAMIN E ON BIOMARKERS OF OXIDATIVE STRESS AND INFLAMMATORY RESPONSE IN A RAT MODEL OF ENDOTOXEMIA

Hussein, S.A. a, El-Senosi, Y.A. a, Omnia M. Abd El-Hamid a and Bakr, W.M.T.

Abstract

The present study aimed to investigate the protective effect of vitamin E on the oxidative stress induced by lipopolysaccharide (LPS, endotoxin) in rats. Male rats (n=80) were divided into four equal groups. Group I (control): rats administered corn oil (20 mg/kg/b.w/day) for 5 weeks. Group II, rats administered vitamin E orally (100 mg/kg/b.w/day) for 5 weeks. Group III, rats injected intraperitoneally (I/P) with LPS (20 mg/kg/b.w.). Group IV, rats administered vitamin E (100 mg/kg/b.w/day) for 5 weeks and then I/P with LPS (20 mg/kg/b.w.). Serum and liver samples were collected at 2 and 5 hours post-injection with endotoxin. Sera were assessed for glucose, uric acid, total cholesterol, triacylglycerols (TG), phospholipids, free fatty acids [FFA], nitric oxide (NO), aspartate aminotransferase (AST), alanine aminotransferase (ALT), ɤ-glutamyltransferase (GGT), haptoglobin, α-1-acid glycoprotein (α-1-AGP), vitamin C, vitamin E, reduced glutathione (GSH) and L-malondialdehyde (L-MDA) in addition for determination of liver glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), vitamin C, vitamin E, GSH and L-MDA. The obtained results revealed that, endotoxemia potentially increased serum glucose, uric acid, TG, phospholipid, FFA, haptoglobin, AGP, GSH, L-MDA concentrations as well as ALT, AST and GGT activities. Moreover, the values of SOD, CAT activities and GSH, L-MDA concentrations in liver tissues were significantly decreased. Treatment with vitamin E decreased LPStriggered pathogenic responses by mitigating liver damage and prevented the increase of L-MDA, glucose, uric acid, triacylglycerols, phospholipid, FFA, ALT, GGT, haptoglobin and AGP. Moreover, treatment with vitamin E in endotoxin injected rats ameliorates the oxidative stress by increasing the concentrations of serum and tissue GSH, SOD, CAT and Vitamin C. It could be concluded that, vitamin E protects against lipid peroxidation, oxidative stress and decrease the inflammatory response to endotoxin injection.