Abstract of research

PREPARATION AND EVALUATION OF COMBINED INACTIVATED RESPIRATORY VIRAL VACCINE PNEUMO-3 BY USING OF MONTANIDE OIL ISA 206 AS AN ADJUVANT

El-Bagoury, G.F.a, El-Sabbagh,M.M b and El-Hawary,R.I. b

Abstract

The present study aimed for preparation of combined inactivated vaccine containing bovine viral diarrhoea virus, infectious bovine rhinotracheitis and parainfluenza-3 viruses adjuvant with Montanide oil ISA 206. The prepared vaccine was evaluated for purity, safety and potency tests. The study proved that the prepared vaccine adjuvanted with Montanide oil ISA 206 was pure and completely safe to be used in calves without any local or systemic post-vaccinal reaction. Potency test was performed on two groups of calves four for each group, first group was vaccinated with combined inactivated respiratory viral vaccine adjuvant with Montanide oil ISA 206 and the second group was left as non-vaccinated control group. Explaining of humoral immune response by using of serum neutralization test revealed that the serum neutralizing antibody titres were developed more higher than the minimal acceptable titre of protective level at one month post vaccination and lasts for 12 months post vaccination to IBRV, 9 months to BVDV and 10 months to PI-3V. In conclusion, the prepared combined inactivated vaccine being pure, completely safe and perfectly potent and effective control of pneumo-enteritis disease complex syndrome.