COMPARISON OF THREE DIAGNOSTIC TECHNIQUES FOR DETECTING BOVINE VIRUS DIARRHEA VIRUS (BVDV) IN BUFFY COAT SAMPLES

El-Bagoury, G.F.a, El-Nahas, E.M.a, Rbab. T.M. Hassanien b and Nawal. M.A. Youssef. b

Abstract

Three diagnostic techniques were compared in terms of their ability to detect BVDV in Buffy coats. Two hundred twenty seven BVDV Buffy coats were used. The techniques used were antigen captures ELISA (ACE), virus isolation-immunoflourescent test (IFX), and one step real time reverse transcription-polymerase chain reaction (RRT-PCR). Out of 227 Buffy coat samples, 35 (15.4%) were found to be positive with RRT-PCR, 23 (10.1%) with ACE, and 20 (8.8%) with virus isolation-IFT. The results obtained indicated that RRT-PCR is more suitable for the detection of BVDV in Buffy coats when compared to other techniques, and ACE can be used for the routine diagnosis of BVDV infection as an alternative technique to virus isolation-IFT

Key words

BVDV, ACE, IFX, RRT-PCR