COMPARATIVE EVALUATION OF INACTIVATED RIFT VALLEY FEVER VIRUS VACCINE ADJUVANTED WITH ALUMINUM PHOSPHATE AND ALUMINUM HYDROXIDE GEL

El-Bagoury, G.F.a, El-Nahas, E.M.a , Marwa Y. Hammad b and Lily S. Salama b

Abstract

Inactivated tissue culture adapted Rift Valley Fever (RVF) Virus vaccines were prepared using Aluminum phosphate and Aluminum hydroxide gel. The prepared vaccines were tested for stability and the humeral immune response in sheep using SNT and ELISA after a single dose. RVF-alum. Phosphate vaccine was valid at 37°C for 2 days, room temperature for 5 days and 4°C for 8month while RVF-alum. Hydroxide was valid at 37°C for 2 days, room temperature for 4 days and 4°C for 6 month. Protective serum antibody titers for aluminum phosphate gel adjuvant RVFV vaccine started at 2nd week post vaccination and persisted till the 8th month then declined under the protective titer for both SNT and ELISA. Protective serum antibody titers for Aluminum hydroxide gel adjuvant RVF virus vaccine started at 2nd week post vaccination and persisted till the 6th month then declined under the protective titer for both SNT and ELISA. The results revealed that aluminum phosphate RVFV vaccine was more stable and elicited early protective immune response with longer duration than aluminum hydroxide RVF virus vaccine

Key words

Adjuvant, Alum, ELISA, RVF vaccine, SNT