ISOLATION AND CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS FROM OSTRICHES (STRUTHIO CAMELUS) IN EGYPT

Elboraay, I.M.a, Elshorbagy, M.A.a, Hassan, M.K.b and Hemat S. El-sayedb

Abstract


Newcastle disease (ND) is a highly contagious disease of poultry that causes high mortality in poultry of all ages as well as in young ostriches. Therefore, this study was designed in order to explore ND in ostriches in Egypt. Samples from died ostriches were collected from different farms in five localities (AL-I smailia, AL-Behera, AL-Sharkia, Helwan and Cairo) and were examined by inoculation in SPF embryonated chicken eggs. Newcastle disease virus (NDV) was isolated from kidney samples collected from AL-Ismailia farm (13/23). Negative NDV isolation was recorded from other farms. Interestingly, Intracerebral pathogenicity index (ICPI) of NDV isolate was 0.8 that indicated virulence of this virus while the isolate had the lentogenic motif 111G-R-N-Q-G-R-L117 at the cleavage site of fusion protein. To the best of our knowledge, this study seems to be the first report of NDV isolation from ostriches in Egypt.

Key words


F protein Cleavage site, ICPI, kidney, Newcastle disease virus (NDV), Ostrich.

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